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mtfp sequence  (Addgene inc)


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    Addgene inc mtfp sequence
    Mtfp Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mtfp sequence/product/Addgene inc
    Average 93 stars, based on 2 article reviews
    mtfp sequence - by Bioz Stars, 2026-05
    93/100 stars

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    Fig. 3. Blue light-induced clustering of FL-OptoSTING and its subcellular localization. (A) Schematic of mCherry fused αGFP-CRY2 construct and FP-fused FL- OptoSTING construct. (B) Fluorescence images of HeLa cells co-expressing an ER marker with either STING(1−378)-EYFP or EYFP-STING(1−378). (C) Fluorescence images of HeLa cells co-expressing #7 (αGFP-mCherry-CRY2) and #8 (EYFP-STING(1−378)). Cells were illuminated by blue light for 3 min at 20-s intervals. Power density of light was fixed at 1 mW mm−2. Enlarged images of indicated white box in right bottom. (D) Fluorescence images of HeLa cells expressing #8 with either <t>mTFP1-fused</t> Golgi or ER marker. (E) Analysis of co-localization of EYFP-STING(1−378) and indicated organelle markers from D. Graph represents the mean and SD. Scale bars, 20 µm in B, C, and D and 2 µm in enlarged image in C, respectively.
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    Fig. 3. Blue light-induced clustering of FL-OptoSTING and its subcellular localization. (A) Schematic of mCherry fused αGFP-CRY2 construct and FP-fused FL- OptoSTING construct. (B) Fluorescence images of HeLa cells co-expressing an ER marker with either STING(1−378)-EYFP or EYFP-STING(1−378). (C) Fluorescence images of HeLa cells co-expressing #7 (αGFP-mCherry-CRY2) and #8 (EYFP-STING(1−378)). Cells were illuminated by blue light for 3 min at 20-s intervals. Power density of light was fixed at 1 mW mm−2. Enlarged images of indicated white box in right bottom. (D) Fluorescence images of HeLa cells expressing #8 with either mTFP1-fused Golgi or ER marker. (E) Analysis of co-localization of EYFP-STING(1−378) and indicated organelle markers from D. Graph represents the mean and SD. Scale bars, 20 µm in B, C, and D and 2 µm in enlarged image in C, respectively.

    Journal: Sensors and Actuators B: Chemical

    Article Title: Optogenetic STING clustering system through nanobody-fused photoreceptor for innate immune regulation

    doi: 10.1016/j.snb.2023.134822

    Figure Lengend Snippet: Fig. 3. Blue light-induced clustering of FL-OptoSTING and its subcellular localization. (A) Schematic of mCherry fused αGFP-CRY2 construct and FP-fused FL- OptoSTING construct. (B) Fluorescence images of HeLa cells co-expressing an ER marker with either STING(1−378)-EYFP or EYFP-STING(1−378). (C) Fluorescence images of HeLa cells co-expressing #7 (αGFP-mCherry-CRY2) and #8 (EYFP-STING(1−378)). Cells were illuminated by blue light for 3 min at 20-s intervals. Power density of light was fixed at 1 mW mm−2. Enlarged images of indicated white box in right bottom. (D) Fluorescence images of HeLa cells expressing #8 with either mTFP1-fused Golgi or ER marker. (E) Analysis of co-localization of EYFP-STING(1−378) and indicated organelle markers from D. Graph represents the mean and SD. Scale bars, 20 µm in B, C, and D and 2 µm in enlarged image in C, respectively.

    Article Snippet: Chemical 399 (2024) 134822 (Clontech) was PCR-amplified using mCherry-F and mCherry-R and inserted between αGFP and CRY2 at the AgeI site using InFusion cloning. mTFP1 sequence from mTFP1-C1 (Addgene plasmid #54613) was digested via AgeI and BsrGI and ligated into pmTurquoise2-ER (Addgene plasmid #36204) and pmTurquoise2-Golgi (Addgene plasmid #36205) after the excision of mTurquoise2 to generate mTFP1-ER and mTFP1-Golgi, respectively.

    Techniques: Construct, Fluorescence, Expressing, Marker